anti il1 β Search Results


immunohistochemical interleukin il  (Servicebio Inc)
86
Servicebio Inc immunohistochemical interleukin il
Histology and <t>IL-2</t> expression in rat hemorrhoidal tissue. A Microscopic appearance of the hemorrhoid areas of the rats in each group (HE, ×50; a model group, b control group). B Expression of IL-2 in anorectal tissue in each group detected by immunohistochemical staining. (IHC, ×50; a model group, b control group)
Immunohistochemical Interleukin Il, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il1+%CE%B2/1+anti+il+%CE%B2/pmc13031196-64-85-90
Average 86 stars, based on 1 article reviews
immunohistochemical interleukin il - by Bioz Stars, 2026-10
86/100 stars
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monoclonal hamster anti-il-1 β antibody  (Becton Dickinson)
90
Becton Dickinson monoclonal hamster anti-il-1 β antibody
Percentage of keratinocytes expressing CXCL1/2 ( a ), TNF- α ( b ), <t>IL-1</t> β ( c ) and epithelial cells expressing CXCL1/2 ( d ) in control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( e ) Transwell migration assay with control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( f ) Numbers of infected CXCL1/2 and IL-1 β positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection ( g ) Numbers of uninfected CXCL1/2 and IL-1 β -positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection. Each bar represents the mean from three experiments ( N =3)±S.E.M. * Represents significant differences with P ≤0.05, whereas ** P ≤0.001
Monoclonal Hamster Anti Il 1 β Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il1+%CE%B2/monoclonal+hamster+anti+il+1+%CE%B2+antibody/pmc03101811-122-33-38
Average 90 stars, based on 1 article reviews
monoclonal hamster anti-il-1 β antibody - by Bioz Stars, 2026-10
90/100 stars
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anti-il-1 β  (AbSci LLC)
90
AbSci LLC anti-il-1 β
Percentage of keratinocytes expressing CXCL1/2 ( a ), TNF- α ( b ), <t>IL-1</t> β ( c ) and epithelial cells expressing CXCL1/2 ( d ) in control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( e ) Transwell migration assay with control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( f ) Numbers of infected CXCL1/2 and IL-1 β positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection ( g ) Numbers of uninfected CXCL1/2 and IL-1 β -positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection. Each bar represents the mean from three experiments ( N =3)±S.E.M. * Represents significant differences with P ≤0.05, whereas ** P ≤0.001
Anti Il 1 β, supplied by AbSci LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il1+%CE%B2/anti+il+1+%CE%B2/pmc09020982-46-23-27
Average 90 stars, based on 1 article reviews
anti-il-1 β - by Bioz Stars, 2026-10
90/100 stars
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biotin-conjugated anti-il-1 β  (NeoBioscience Technology Co Ltd)
90
NeoBioscience Technology Co Ltd biotin-conjugated anti-il-1 β
Percentage of keratinocytes expressing CXCL1/2 ( a ), TNF- α ( b ), <t>IL-1</t> β ( c ) and epithelial cells expressing CXCL1/2 ( d ) in control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( e ) Transwell migration assay with control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( f ) Numbers of infected CXCL1/2 and IL-1 β positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection ( g ) Numbers of uninfected CXCL1/2 and IL-1 β -positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection. Each bar represents the mean from three experiments ( N =3)±S.E.M. * Represents significant differences with P ≤0.05, whereas ** P ≤0.001
Biotin Conjugated Anti Il 1 β, supplied by NeoBioscience Technology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il1+%CE%B2/biotin+conjugated+anti+il+1+%CE%B2/pmc06189688-60-55-63
Average 90 stars, based on 1 article reviews
biotin-conjugated anti-il-1 β - by Bioz Stars, 2026-10
90/100 stars
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anti–il-1 β rabbit primary antibody  (ImmunoWay Biotechnology Company)
90
ImmunoWay Biotechnology Company anti–il-1 β rabbit primary antibody
Percentage of keratinocytes expressing CXCL1/2 ( a ), TNF- α ( b ), <t>IL-1</t> β ( c ) and epithelial cells expressing CXCL1/2 ( d ) in control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( e ) Transwell migration assay with control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( f ) Numbers of infected CXCL1/2 and IL-1 β positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection ( g ) Numbers of uninfected CXCL1/2 and IL-1 β -positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection. Each bar represents the mean from three experiments ( N =3)±S.E.M. * Represents significant differences with P ≤0.05, whereas ** P ≤0.001
Anti–Il 1 β Rabbit Primary Antibody, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il1+%CE%B2/anti+il+1+%CE%B2+rabbit+primary+antibody/pmc09095358-70-31-27
Average 90 stars, based on 1 article reviews
anti–il-1 β rabbit primary antibody - by Bioz Stars, 2026-10
90/100 stars
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il-1 beta polyclonal antibody  (Bioss)
95
Bioss il-1 beta polyclonal antibody
Percentage of keratinocytes expressing CXCL1/2 ( a ), TNF- α ( b ), <t>IL-1</t> β ( c ) and epithelial cells expressing CXCL1/2 ( d ) in control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( e ) Transwell migration assay with control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( f ) Numbers of infected CXCL1/2 and IL-1 β positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection ( g ) Numbers of uninfected CXCL1/2 and IL-1 β -positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection. Each bar represents the mean from three experiments ( N =3)±S.E.M. * Represents significant differences with P ≤0.05, whereas ** P ≤0.001
Il 1 Beta Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il1+%CE%B2/IL-1+Beta+Polyclonal+Antibody/custom%40bs-6319r%4030408497
Average 95 stars, based on 1 article reviews
il-1 beta polyclonal antibody - by Bioz Stars, 2026-10
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anti il 18  (Affinity Biosciences)
86
Affinity Biosciences anti il 18
Percentage of keratinocytes expressing CXCL1/2 ( a ), TNF- α ( b ), <t>IL-1</t> β ( c ) and epithelial cells expressing CXCL1/2 ( d ) in control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( e ) Transwell migration assay with control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( f ) Numbers of infected CXCL1/2 and IL-1 β positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection ( g ) Numbers of uninfected CXCL1/2 and IL-1 β -positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection. Each bar represents the mean from three experiments ( N =3)±S.E.M. * Represents significant differences with P ≤0.05, whereas ** P ≤0.001
Anti Il 18, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il1+%CE%B2/1+anti+il+%CE%B2/pm41833150-104-36-49
Average 86 stars, based on 1 article reviews
anti il 18 - by Bioz Stars, 2026-10
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p17 antibody  (Abmart Inc)
86
Abmart Inc p17 antibody
Percentage of keratinocytes expressing CXCL1/2 ( a ), TNF- α ( b ), <t>IL-1</t> β ( c ) and epithelial cells expressing CXCL1/2 ( d ) in control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( e ) Transwell migration assay with control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( f ) Numbers of infected CXCL1/2 and IL-1 β positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection ( g ) Numbers of uninfected CXCL1/2 and IL-1 β -positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection. Each bar represents the mean from three experiments ( N =3)±S.E.M. * Represents significant differences with P ≤0.05, whereas ** P ≤0.001
P17 Antibody, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il1+%CE%B2/1+anti+il+p17+%CE%B2/pmc13130659-9-3-6
Average 86 stars, based on 1 article reviews
p17 antibody - by Bioz Stars, 2026-10
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il-1 alpha polyclonal antibody  (Bioss)
94
Bioss il-1 alpha polyclonal antibody
Percentage of keratinocytes expressing CXCL1/2 ( a ), TNF- α ( b ), <t>IL-1</t> β ( c ) and epithelial cells expressing CXCL1/2 ( d ) in control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( e ) Transwell migration assay with control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( f ) Numbers of infected CXCL1/2 and IL-1 β positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection ( g ) Numbers of uninfected CXCL1/2 and IL-1 β -positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection. Each bar represents the mean from three experiments ( N =3)±S.E.M. * Represents significant differences with P ≤0.05, whereas ** P ≤0.001
Il 1 Alpha Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il1+%CE%B2/IL-1+Alpha+Polyclonal+Antibody/custom%40bs-4947r%4027521968
Average 94 stars, based on 1 article reviews
il-1 alpha polyclonal antibody - by Bioz Stars, 2026-10
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Image Search Results


Histology and IL-2 expression in rat hemorrhoidal tissue. A Microscopic appearance of the hemorrhoid areas of the rats in each group (HE, ×50; a model group, b control group). B Expression of IL-2 in anorectal tissue in each group detected by immunohistochemical staining. (IHC, ×50; a model group, b control group)

Journal: Techniques in Coloproctology

Article Title: Comparison of the modeling effects between two hemorrhoid models

doi: 10.1007/s10151-026-03303-x

Figure Lengend Snippet: Histology and IL-2 expression in rat hemorrhoidal tissue. A Microscopic appearance of the hemorrhoid areas of the rats in each group (HE, ×50; a model group, b control group). B Expression of IL-2 in anorectal tissue in each group detected by immunohistochemical staining. (IHC, ×50; a model group, b control group)

Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

Techniques: Expressing, Control, Immunohistochemical staining, Staining

Immunohistochemical overview of anorectal cytokine expression. Expression of A IL-2, B IL-6, C , and D TNFα in anorectal tissue in each group detected by immunohistochemical staining (IHC, ×50; a model group, b control group)

Journal: Techniques in Coloproctology

Article Title: Comparison of the modeling effects between two hemorrhoid models

doi: 10.1007/s10151-026-03303-x

Figure Lengend Snippet: Immunohistochemical overview of anorectal cytokine expression. Expression of A IL-2, B IL-6, C , and D TNFα in anorectal tissue in each group detected by immunohistochemical staining (IHC, ×50; a model group, b control group)

Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

Techniques: Immunohistochemical staining, Expressing, Staining, Control

Relative mRNA expression of pro-inflammatory cytokines A IL-2, B IL-6, and C TNFα in anorectal tissue in each group. * P < 0.05 vs control group

Journal: Techniques in Coloproctology

Article Title: Comparison of the modeling effects between two hemorrhoid models

doi: 10.1007/s10151-026-03303-x

Figure Lengend Snippet: Relative mRNA expression of pro-inflammatory cytokines A IL-2, B IL-6, and C TNFα in anorectal tissue in each group. * P < 0.05 vs control group

Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

Techniques: Expressing, Control

Western blotting for detecting protein expression of IL-2, IL-6, and TNFα

Journal: Techniques in Coloproctology

Article Title: Comparison of the modeling effects between two hemorrhoid models

doi: 10.1007/s10151-026-03303-x

Figure Lengend Snippet: Western blotting for detecting protein expression of IL-2, IL-6, and TNFα

Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

Techniques: Western Blot, Expressing

IL-2, IL-6, and TNFα protein levels in anorectal tissue are significantly elevated in the model group compared to the control group (* P < 0.05). A IL-2, B IL-6, and C TNFα protein expression in anorectal tissue. * P < 0.05 vs control group

Journal: Techniques in Coloproctology

Article Title: Comparison of the modeling effects between two hemorrhoid models

doi: 10.1007/s10151-026-03303-x

Figure Lengend Snippet: IL-2, IL-6, and TNFα protein levels in anorectal tissue are significantly elevated in the model group compared to the control group (* P < 0.05). A IL-2, B IL-6, and C TNFα protein expression in anorectal tissue. * P < 0.05 vs control group

Article Snippet: The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326).

Techniques: Control, Expressing

Percentage of keratinocytes expressing CXCL1/2 ( a ), TNF- α ( b ), IL-1 β ( c ) and epithelial cells expressing CXCL1/2 ( d ) in control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( e ) Transwell migration assay with control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( f ) Numbers of infected CXCL1/2 and IL-1 β positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection ( g ) Numbers of uninfected CXCL1/2 and IL-1 β -positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection. Each bar represents the mean from three experiments ( N =3)±S.E.M. * Represents significant differences with P ≤0.05, whereas ** P ≤0.001

Journal: Cell Death & Disease

Article Title: Role of Fas/FasL in regulation of inflammation in vaginal tissue during HSV-2 infection

doi: 10.1038/cddis.2011.14

Figure Lengend Snippet: Percentage of keratinocytes expressing CXCL1/2 ( a ), TNF- α ( b ), IL-1 β ( c ) and epithelial cells expressing CXCL1/2 ( d ) in control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( e ) Transwell migration assay with control and HSV-2-infected keratinocyte cell cultures at 18 h p.i. exposed or not to anti-Fas cytotoxic antibody (10 μ g/ml) and anti-FasL blocking antibody (10 μ g/ml). ( f ) Numbers of infected CXCL1/2 and IL-1 β positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection ( g ) Numbers of uninfected CXCL1/2 and IL-1 β -positive cells per 100 cells found at infected sites in the HSV-2-infected Fas (−/−), FasL (−/−) and WT (C57BL/6) mice at 3 days of infection. Each bar represents the mean from three experiments ( N =3)±S.E.M. * Represents significant differences with P ≤0.05, whereas ** P ≤0.001

Article Snippet: TNF- α , CXCL1/2 and IL-1 β were detected using Cytofix/Cytoperm fixation/permeabilization kit with Golgi plug (BD Biosciences) as described above, using polyclonal goat anti-GRO α / β (CXCL1/2) antibody (Santa Cruz Biotechnology), monoclonal hamster anti-IL-1 β antibody (BD Biosciences) and Alexa Fluor 488-conjugated monoclonal rat anti-TNF- α antibody (BD Biosciences).

Techniques: Expressing, Infection, Blocking Assay, Transwell Migration Assay